A Smrt® Approach for Finishing Plant and Animal Genomes

www.pacb.com/denovo In the days of Sanger sequencing, organisms considered most scientifically important were targeted for high-quality, finished genomes. There was no question that this was an expensive and laborious goal, but it was widely accepted that a finished genome answered questions that no draft assembly ever could. As short-read sequencing technologies became available, they were embraced for their cost-effectiveness — but genome finishing, which was virtually impossible with such short reads, was a necessary casualty.